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Stephen P Robinson, MD, Ph.D., and Andrew Stagg, Ph.D., have brought together a wide range of time-proven methods for studying these dendritic cells.
Many of these readily reproducible techniques deal with the problem of obtaining sufficient dendritic cells for analysis, whether by isolation from a wide variety of tissues or from various progenitor cell populations.
Other methods described in a step-by-step fashion the techniques commonly used for analyzing aspects of dendritic cells, ranging from cell migration to antigen uptake and T cell stimulation.
In addition, a few techniques explore the practical challenges involved in using dendritic cells in a clinical setting to develop novel immunotherapeutics.
Arindam Maiti
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