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This book aims to provide a comprehensive understanding of the interaction between the matter and electromagnetic radiation, emphasizing its application in drug analysis. It covers UV-visible spectroscopy, focusing on instrumentation and its use in spectrophotometric titrations. The study extends to fluorimetry and IR spectroscopy, including key factors affecting IR spectra and the use of specialized instruments like Golay cells and thermistors. Techniques such as flame photometry, atomic absorption spectroscopy, and nepheloturbidometry are explored for their pharmaceutical applications.
An introduction to chromatography highlights adsorption and partition principles, particularly in column chromatography. The course also delves into the principles and techniques of thin layer chromatography (TLC) and paper chromatography. Electrophoresis, including various types like capillary electrophoresis, is discussed in detail. Further, the text covers gas chromatography (GC) and high performance liquid chromatography (HPLC), focusing on instrumentation and derivatisation methods. Ion exchange chromatography is introduced with emphasis on resin properties and methodologies. It also includes gel chromatography and affinity chromatography, highlighting their roles in separation and purification processes.
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